Table 2 Measurement of size and heterogeneity of recombinant refolded nitrilase in fractions separated by gel filtration (see Figure 2) using dynamic light scattering (DLS)
From: Heterologous expression, purification and characterization of nitrilase from Aspergillus nigerK10
Elution time, min | Diameter, nm | Mw, kDa | PdI |
|---|---|---|---|
32 | 22.8 | 1000 | 0.30 |
34 | 19.7 | 710 | 0.24 |
36 | 17.0 | 500 | 0.29 |
38 | 14.5 | 350 | 0.23 |
40 | 14.8 | 370 | 0.23 |