Table 1 Purification of recombinant nitrilase from Aspergillus niger K10
From: Heterologous expression, purification and characterization of nitrilase from Aspergillus nigerK10
Step | Total protein, mg | Specific activity, U mg -1 | Total activity, U | Yield, % | Purification, fold |
|---|---|---|---|---|---|
Cell-free extract | 444.4 | 0.29 | 130.5 | 100 | - |
Q-Sepharose | 159.5 | 0.39 | 61.5 | 43.2 | 1.34 |
Sephacryl S-200 | 76.7 | 0.60 | 46.1 | 35.1 | 2.07 |