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Table 1 Purification of recombinant nitrilase from Aspergillus niger K10

From: Heterologous expression, purification and characterization of nitrilase from Aspergillus nigerK10

Step Total protein,
mg
Specific activity,
U mg -1
Total activity,
U
Yield,
%
Purification,
fold
Cell-free extract 444.4 0.29 130.5 100 -
Q-Sepharose 159.5 0.39 61.5 43.2 1.34
Sephacryl S-200 76.7 0.60 46.1 35.1 2.07
  1. Enzyme activity was assayed with 25 mM benzonitrile (see Methods for details).