Figure 3

Multiple sequence alignment. (A) Multiple sequence alignment of A. niger K10 with template structures 1EMS [21], 1J31 [22], 1F89 [23], 2VHH [24] and 2W1V [25] (letters in upper case) and sequences of homologous nitrilases (letters in lower case) from Rhodococcus rhodochrous J1 (RrJ1) [12] and from Pseudomonas fluorescens (Pf-5) [1]. Clustal W scheme is used for marking similar residues. Amino acids from catalytic triad are strongly conserved; they are marked by red arrow and enclosed in blue rectangles. Secondary structures for template 1EMS and A. niger K 10 nitrilase model are shown above and under the aligned sequences, respectively, with numeration as in [12]. (B) Sequence alignment of A. niger K 10 with 2KIN [26] was used for modelling the C-terminal part. Secondary structure as assigned by Procheck [27] is shown for A. niger K 10 and for 2KIN above and under the aligned sequences, respectively.