Figure 1

Summary of the sequence analysis of heterologously expressed nitrilase vs . nitrilase isolated from the native organism (Nit-ANigRec and Nit-ANigWT respectively). Sequence analysis was performed by automated Edman degradation of nitrilase blotted onto PVDF membrane (underlined) in combination with peptide mass mapping using MALDI TOF mass spectrometry of peptides extracted after in gel digestion with trypsin (bold) or Asp-N protease (italics). The position of C-terminal truncation by 46 amino acids in the native preparation is indicated by an asterisk.