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Figure 4 | BMC Biotechnology

Figure 4

From: Measurement of diffusion in articular cartilage using fluorescence correlation spectroscopy

Figure 4

Summary of the FCS measurements. a. Representative of auto correlation curves for Normal, Trypsin-3, Trypsin-6, Trypsin-9, and trypsin-24 b. Diffusion coefficients of Alexa-albumin, E-10 = ×10-10. FCS data demonstrated that diffusion coefficients of Alexa Fluor 488 conjugated with albumin (Alexa-albumin) were greater when measuring degenerated cartilage, indicating that FCS probes moved more freely in degenerated cartilage than in normal tissue. Interestingly, diffusion coefficients for trypsin-3 and trypsin-6 were lower than in normal cartilage. p values of each of groups were under 0.01 which demonstrated the significance of the difference between the groups excluding three of p values between the groups; HA vs.Trypsin-24, Normal vs. Trypsin-9, and Trypsin-3 vs. Trypsin-6. Among these higher values over 0.01, p value between No significant difference was found between Trypsin-3 and Trypsin-6(p = 0.486). Besides, we found a significant difference in the rest of two values which were below 0.05 (p = 0.042; between HA and Trypsin-24, and p = 0.021; Normal and Trypsin-9).

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