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Table 3 Summary of parameter values used for model simulations.*

From: Effects of a recombinant gene expression on ColE1-like plasmid segregation in Escherichia coli

Parameter

Value

Units

D

0.3

h-1

Ginmax

0.036

g plasmid DNA/L biomass

Ks

0.005

g/L

kd

0.46

min-1

ke

0

min-1

kp0

2400/(233 μ-2 + 78)

min-1**

kq0

3600α/(82.5 μ-1 + 145)

min-1**

 

α = 1 for μ > ln2

 
 

α = μ/ln2 for μ < ln2***

 

m

2.399****

-

Np

41 (pP1-(SD)-hIFNγ)

-

 

24 (pP1-(4SD)-hIFNγ)

 
 

30 (pP1-(ΔSD)-hIFNγ)

 

n

1

-

pinmax

150

g protein/L dry biomass

sF

1.96

g/L

μmax

0.66

h-1

ρB

1000

g dry biomass/L dry biomass

  1. *In the absence of translation (as in pP1-(ΔSD)-hIFNγ) the gene expression parameters are ignored.
  2. **Calculating k p 0 and k q 0 the specific growth rate μ is expressed in h-1.
  3. ***By α = μ/ln2 (for μ < ln2) and Eq. (9), the substitution of k p 0, k q 0 and k d in Eq. 11 results in Eq. 15.
  4. ****The m value was determined by nonlinear fitting for pP1-(ΔSD)-hIFNγ and set in the calculations for both pP1-(SD)-hIFNγ and pP1-(4SD)-hIFNγ (see below).