Figure 1
From: Detection of NASBA amplified bacterial tmRNA molecules on SLICSel designed microarray probes
Non-specific microarray signal intensities from hybridization experiments with target and five control tmRNAs. Non-specific tmRNA signal intensities are divided by the corresponding probe-specific signal intensity. Target hybridization signal intensity is given on a y-axis as a 100% signal baseline. A maximum false-positive signal threshold is shown as horizontal 10% dotted line. Microarray signals are distributed along x-axis according to the calculated binding energy difference between the specific and non-specific binding (ΔGtarget - ΔGcontrol (ΔΔG) 0.2...10.7 kcal/mol). Dotted vertical lines separate the probes with binding energy difference smaller then 2 and 4 kcal/mol, respectively.