Figure 9
From: Identification of gliadin-binding peptides by phage display
Western blot analysis of peptide-gliadin binding. 10 μg of gliadin extracted from Sigma gluten (A) or 10 μg of gliadin extracted from Surco wheat (B) was loaded on a polyacrylamide gel. Separated proteins were transferred to a nitrocellulose membrane. Each membrane was incubated with 1.67 nmoles (1015 molecules) of the peptide indicated at the bottom of the figure. After washing the biotin signal from the bound peptide was developed as described.