Figure 6
From: Identification of gliadin-binding peptides by phage display
Inhibition of gliadin/anti-gliadin antibody binding by selected synthetic peptides. Increasing amounts of gliadin in ng (as indicated on the X-axis) were incubated with 1.67 nmoles of biotin-labelled synthetic peptides (1015 molecules) for 1 h at room temperature. Buffer (0.1 M NaHCO3) without peptides was used as a control. The mixes were then added to anti-gliadin monoclonal antibody-coated wells provided with the Immunotech gliadin ELISA kit. After incubation for 1 h at room temperature, gliadin-antibody complexes immobilised in the wells were quantified as described (see Methods) and given as absorbance units at 450 nm (Y-axis). Error bars show the variation in three different experiments.