Figure 2

Quantification of immobilised semi-purified gliadin (Sigma). Microtiter wells were coated with increasing amounts of gliadin (ng) as indicated on X-axis. Commercial anti-gliadin antibodies bound to the coated gliadin were quantified by adding a secondary AP-tagged goat anti-human IgG antibody and measured as absorbance at 405 nm (Y-axis).A. Gliadin dissolved in 100 μl 0.1 M NaHCO₃ (pH 8.6).B. Gliadin dissolved in 100 μl 2 M urea.Error bars indicate variation between two different experiments