A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination

Table 1 Evaluation of the xylanasic inhibition activity of CaclXIP towards enzymes of Aspergillus niger and Acrophialophora nainiana

Treatment	Xylanase activity (Abs 540 nm)	Inhibition (%)
A. niger xylanases	0.365 ± 0.057	Not applicable
A. niger xylanases + CaclXIP	0.294 ± 0.010	19.5 ± 3.4
A. nainiana xylanases	0.211 ± 0.018	Not applicable
A. nainiana xylanases + CaclXIP	0.092 ± 0.009	57 ± 9

* Xylanases from A. niger and A. nainiana (60 μg) were incubated with CaclXIP (5 μg) (enzymes:inhibitor ratio 12:1 [w/w]) as described in Material and Methods section. Xylanase activity and percentage inhibition are expressed as means + S. D., n = 3.

ISSN: 1472-6750