Table 2 Transduction efficiency* of human blood monocyte-derived macrophages using different viral systems
| Â | Transduction efficiency | MOI | Viral system | Differentiation Method | Quantification technique |
|---|---|---|---|---|---|
Haddada H, et al. [13] | 40-80% | 100 | Adenovirus | HS | Beta-gal activity |
Huang S, et al. [30] | 52% | 1000 | Adenovirus | M-CSF+FBS | Beta-gal activity |
Schneider SD, et al. [33] | 20% | 100 | Adenovirus | HS | LacZ activity |
De S, et al. [31] | 10% | 100 | Adenovirus | GM-CSF+FBS | Beta-gal activity |
Foxwell B, et al. [12] | > 90% | 100 | Adenovirus | M-CSF+FBS | Beta-gal activity flow cytometry |
Heider H, et al. [14] | 80% | 30 | Adenovirus | HS | GFP fluorescence microscopy |
White S, et al. [34] | 15% | 1 | HIV/SIV based lentivirus | GM-CSF+HS | GFP fluorescence microscopy |
Schroers R, et al. [20] | 30% | 100 | HIV based lentivirus | GM-CSF+FBS | YFP flow cytometry |
Neil S, et al. [19] | 20% | 10 | HIV based lentivirus | HS | GFP flow cytometry |
Ravot E, et al. [32] | 40% | 0.65 | HIV based lentivirus | HS+FBS | GFP flow cytometry |
Lu Y, et al. [21] | 40% | 50 | HIV based lentivirus | HS+FBS | GFP fluorescence microscopy |
Jarrosson-Wuilleme L, et al. [28] | 30% | 25 | HIV based lentivirus | GM-CSF+FBS | GFP flow cytometry |
Zeng L, et al. [29] | 50% | 100 | HIV based lentivirus | HS+FBS | GFP fluorescence microscopy |
Current report | 80-90% | 10 | HIV based lentivirus | M-CSF+FBS | EGFP flow cytometry |