Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: Evaluation of transduction efficiency in macrophage colony-stimulating factor differentiated human macrophages using HIV-1 based lentiviral vectors

Figure 3

Flow cytometry analysis of human blood monocyte-derived macrophages transduced with lentivirus at different MOIs. Cells from donor 2 were transduced with lentivirus VRX494 and VRX1090 two hours after plating using MOIs of 5 (A), 10 (B), 25 (C), and 50 (D) at a final concentration of 3, 6, 15, and 30 × 106 transducing units/ml, respectively. Each bracketed region in the histogram corresponds to % EGFP-positive cells and shaded area corresponds to non-transduced cell control. Flow cytometry was performed on day 8 after cell plating.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us