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Figure 1 | BMC Biotechnology

Figure 1

From: Evaluation of transduction efficiency in macrophage colony-stimulating factor differentiated human macrophages using HIV-1 based lentiviral vectors

Figure 1

EGFP expression and flow cytometry analysis of human blood monocyte-derived macrophages transduced at different times. Cells from donor 1 were transduced with lentivirus VRX1090 using an MOI of 50 (30 × 106 transducing units/ml) at 2 (B), 24 (C), 48 (D) and 120 (E) hours after cell plating. Control non-transduced cells are shown in A. Each row of the upper panel corresponds to the same microscopic field and respective flow cytometry histogram. Photomicrographs were taken on day 8 after cell plating. Scale bar = 50 μm and applies to all. Each bracketed region in the histogram corresponds to % EGFP-positive cells and shaded area corresponds to non-transduced cell control. Flow cytometry was performed on day 9 after cell plating. Shown in lower panel is mean fluorescence intensity per EGFP-positive cell at different times after cell plating.

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