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Table 1 Oligonucleotides employed in this study

From: Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa

Oligonucleotide Size Purpose
Number Sequence (nt)  
Ml-08-5104 5'-TCGAGAAAAG ACATCATCAT CATCATCATA ACCAGGAGCA GGTGTCCCCA CTTACCCTCC TCGCTGGAGG TGGAGGGTAC-3' 80 XhoI-KpnI sense strand for XL1-HSA
ML-08-5105 5'-CCTCCACCTC CAGCGAGGAG GGTAAGTGGG GACACCTGCT CCTGGTTATG ATGATGATGA TGATGTCTTT TC-3' 72 XhoI-KpnI antisense strand for XL1-HSA
ML-08-5106 5- TCGAGAAAAG ACATCATCAT CATCATCATG ATCAGATGAT GCTGCCATGG CCAGCTGTGA CCCTGGGAGG CGGAGGGTAC -3' 80 XhoI-KpnI sense strand for XL2-HSA
ML-08-5107 5- CCTCCGCCTC CCAGGGTCAC AGCTGGCCAT GGCAGCATCA TCTGATCATG ATGATGATGA TGATGTCTTT TC-3' 72 XhoI-KpnI antisense strand for XL2-HSA
ML-08-5108 5'-TCGAGAAAAG ACATCATCAT CATCATCATT GGCAGCATAA AATCGATCTG CCATACAATG GTGCAGGAGG CGGAGGGTAC-3' 80 XhoI-KpnI sense strand for XL3-HSA
ML-08-5109 5'- CCTCCGCCTC CTGCACCATT GTATGGCAGA TCGATTTTAT GCTGCCAATG ATGATGATGA TGATGTCTTT TC-3' 72 XhoI-KpnI antisense strand for XL3-HSA
ML-08-5116 5'- TCGAGAAAAG AAACCAGGAG CAGGTGTCCC CACTTACCCT CCTCGCTGGA GGTGGAGGGT AC -3' 62 XhoI-KpnI sense strand for XL4-HSA
ML-08-5117 5'- CCTCCACCTC CAGCGAGGAG GGTAAGTGGG GACACCTGCT CCTGGTTTCT TTTC-3'. 54 XhoI-KpnI antisense strand for XL4-HSA
ML-08-5118 5'- TCGAGAAAAG AGATCAGATG ATGCTGCCAT GGCCAGCTGT GACCCTGGGA GGCGGAGGGT AC -3' 62 XhoI-KpnI sense strand for XL5-HSA
ML-08-5119 5'- CCTCCGCCTC CCAGGGTCAC AGCTGGCCAT GGCAGCATCA TCTGATCTCT TTTC -3' 54 XhoI-KpnI antisense strand for XL5-HSA
ML-08-5120 5'-TCGAGAAAAG ATGGCAGCAT AAAATCGATC TGCCATACAA TGGTGCAGGA GGCGGAGGGT AC-3' 62 XhoI-KpnI sense strand for XL6-HSA
ML-08-5121 5'-CCTCCGCCTC CTGCACCATT GTATGGCAGA TCGATTTTAT GCTGCCATCT TTTC-3' 54 XhoI-KpnI antisense strand for XL6-HSA
ML-09-3874 5'-ACGTGTCGAGA AAAGACATCA TCATCATCAT CATAACCAGG AGCAGGTGTC CCCAC-3' 45 Upstream primer for PCR to assemble H6α2AP(13-42)-HSA
ML 17225 5'-ACGTGGTACCG ACTCCTGGGG GACTCTTCAG-3' 30 Downstream primer for PCR to assemble H6α2AP(13-42)-HSA