TY - JOUR AU - Sheffield, William P. AU - Eltringham-Smith, Louise J. PY - 2011 DA - 2011/12/20 TI - Incorporation of albumin fusion proteins into fibrin clots in vitro and in vivo: comparison of different fusion motifs recognized by factor XIIIa JO - BMC Biotechnology SP - 127 VL - 11 IS - 1 AB - The transglutaminase activated factor XIII (FXIIIa) acts to strengthen pathological fibrin clots and to slow their dissolution, in part by crosslinking active α2-antiplasmin (α2AP) to fibrin. We previously reported that a yeast-derived recombinant fusion protein comprising α2AP residues 13-42 linked to human serum albumin (HSA) weakened in vitro clots but failed to become specifically incorporated into in vivo clots. In this study, our aims were to improve both the stability and clot localization of the HSA fusion protein by replacing α2AP residues 13-42 with shorter sequences recognized more effectively by FXIIIa. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-11-127 DO - 10.1186/1472-6750-11-127 ID - Sheffield2011 ER -