Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: In situgastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli

Figure 3

Flow cytometry analysis of lactobacilli surface displayed scFvs. (A) Visualisation of the production and display of the 1H scFv on the surface of recombinant L. paracasei presenting the scFv anchored (KKA307) or attached (KKA317) (wt L. paracasei was as a negative control). ScFvs were visualised by detection of the E-tag fused to the scFv using a mouse anti-E-tag antibody in conjunction with a FITC conjugated rabbit anti-mouse immunoglobulin antibody. (B) Binding of recombinant L. paracasei through surface displayed 1H scFv to PA coated fluorescent beads. The display of scFv on surface visualised through binding of mouse anti-E-tag antibody and FITC conjugated rabbit anti-mouse immunoglobulin antibody. 1,1 quarter: unlabeled L. paracasei, 2,1 quarter: scFv displaying L. paracasei, 1,2 quarter: PA conjugated fluorescent beads and 2,2 quarter: PA conjugated beads bound by L. paracasei.

Back to article page