Figure 2

(A) Detection of the scFv expressed by recombinant L. paracasei by immunoblotting. Cell extract (c) of cell wall anchored strain (KKA307), secreted strain (KKA308) and attached strain (KKA317). Culture supernatant (s) from cell wall anchored strain (KKA307), secreted strain (KKA308) and attached strain (KKA317). The expected size of L. paracasei produced scFvs was 57.1, 29.2 and 42.2 KDa for the anchored, secreted and attached constructs respectively. (B) Binding and quantification of anti-PA scFv secreted into the growth media of the recombinant lactobacilli as measured by ELISA, with 1H scFv purified from E. coli as a reference (average of 4 experiments).