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Figure 1 | BMC Biotechnology

Figure 1

From: In situgastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli

Figure 1

Plasmid constructs for expression of the 1H scFv in lactobacilli based on the expression cassette from the apf gene from Lactobacillus crispatus M247. Variations in the anchoring domain and placement of the translational stop codon gives respectively secreted, cell wall anchored or attached production of the scFv. The APF promoter (P), APF signal peptide (blue), APF anchoring domain (light blue), translational stop codon (arrowhead), 1H scFv (white), prtP anchor (yellow), E-tag (orange) and transcriptional terminator (lollipop) are indicated.

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BMC Biotechnology

ISSN: 1472-6750

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