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Table 1 Species quantification results from a survey of selected xenografts.

From: Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts

Xenograft Name Tumor
Type
in vivo Passage Initial DNA (ng) Average % Human ± SD Average
% Mouse
Calculated Human
DNA
Calculated Mouse
DNA
Sum DNA (ng) % of Initial DNA
HL-60(TB) Leukemia P01 27.6 100.0 ± 0.00 0.0 26.96 0.00 27.0 97.7
MDA-MB-361 Breast P01 48.8 12.8 ± 1.84 87.2 4.80 31.20 36.0 73.7
NCI-H157 Lung P01 72.4 98.9 ± 1.50 1.1 97.83 1.29 99.1 136.8
RXF-631 Renal P01 23.2 43.2 ± 0.06 56.8 9.90 11.34 21.2 91.5
SK-MEL-2 Melanoma P01 29.2 81.8 ± 1.42 18.2 38.63 7.66 46.3 158.4
UACC-257 Melanoma P01 53.7 99.9 ± 0.08 0.1 49.49 0.04 49.5 92.3
LOX IMVI Melanoma P01-a 50.6 48.4 ± 7.26 51.6 16.15 17.54 33.7 66.6
LOX IMVI Melanoma P01-b 52.8 55.1 ± 6.38 45.0 19.30 16.77 36.1 68.3
LOX IMVI Melanoma P01-c 55.5 39.6 ± 7.99 60.4 17.13 27.34 44.5 80.2
LOX IMVI Melanoma P01-d 52.1 53.1 ± 6.56 46.9 24.81 35.35 60.2 115.4
LOX IMVI Melanoma P01-e 53.5 33.9 ± 7.76 66.1 20.15 45.56 65.7 122.9
AS283 Lymphoma P04-a 56.9 83.5 ± 5.89 16.5 37.12 7.39 44.5 78.3
AS283 Lymphoma P04-b 71.6 83.6 ± 4.90 16.4 86.85 13.31 100.2 139.8
CA46 Lymphoma P04 43.4 99.9 ± 0.09 0.1 47.76 0.04 47.8 110.2
CP70 Ovarian P04 61.4 83.5 ± 3.23 16.6 59.56 9.87 69.4 113.0
Daudi Lymphoma P04 56.5 100.0 ± 0.02 0.0 49.03 0.01 49.0 86.7
GTL-16 Gastric P04 42.3 100.0 ± 0.00 0.0 39.92 0.00 39.9 94.4
OVCAR-5 Ovarian P04 53 88.8 ± 0.31 11.2 63.11 6.98 70.1 132.1
AS283 Lymphoma P10 49.2 99.3 ± 0.82 0.7 48.04 0.22 48.3 98.1
HuH-7 Liver P10 55.2 90.1 ± 4.26 9.9 52.24 4.36 56.6 102.5
NCI-H1975-Luc-GFP Lung P10 56.4 34.9 ± 6.18 65.1 18.67 29.45 48.1 85.4
NCI-H209-Luc-GFP Lung P10 61.7 81.0 ± 2.00 19.0 79.70 15.83 95.5 154.8
  1. The xenograft names, tumor types, and number of in vivo passages a sample has progressed through are given for each sample at the time of sample collection. Letters following the passage number signify independent tumor samples derived from tumors grown on different mice of the same cohort. After DNA extraction and purification, samples were diluted between 25 ng/μl and 75 ng/μl; one microliter of this dilution serves as the initial DNA (ng) template in the qPCR reactions. The CT value for each sample was derived from standard curves and converted to a log genomes value, which can be converted to the number of measured haploid genomes present. Percent Human and Percent Mouse values were derived by dividing # human genomes by total genomes present or # mouse genomes divided by total genomes present, respectively. Genome numbers for each species are back-calculated into DNA (ng), the sum of which should be equal to the initial DNA used in the qPCR sample. The far right column lists the percentage of the initial sample calculated from the qPCR assay and gives a measure of quality assurance.