Figure 3

Representative example of real-time qPCR fluorescent signal traces (ΔRn vs cycles) showing species specificity of the qPCR probes and a species-specific standard curve derived from each data set. A) The human probe shows robust DNA amplification signals in samples consisting of only LNCaP human prostate carcinoma cell line template DNA. B) In the same LNCaP samples, the mouse probe shows no binding or product amplification. C) Plotting mean CT (+/- SD) vs log human genomes initially present in the qPCR reaction tube, the serial dilution from (A) is linear down to 1.8 human haploid genomes. D) The human-specific probe does not bind to B16F10 mouse melanoma tumor template DNA. E) The mouse-specific probe does show amplification when using B16F10 mouse tumor DNA as a template. F) A standard curve derived from the mean mouse probe CT signal plotted against log mouse genomes present in (E) shows a linear correlation down to 2 initial genomes.