Figure 1

Relevant species alignment using the PTGER2 gene and the PCR primers and real-time qPCR probe sequences derived from the alignment. A) The human and mouse PTGER2 DNA sequences are aligned, with red letters signifying non-conserved basepairs. Human+Mouse PCR primers are listed underneath, and a black bar is below the qPCR probe sequence. The reverse-compliment Common Reverse primer sequence is shown to illustrate alignment on the DNA strand shown. B) Qualitative PCR primers are listed, as well as the expected size of the amplified sequence. All sequences listed 5' to 3'. Mouse genome specific base pairs are underlined in the mouse forward primer and mouse probe sequences. C) Real-time qPCR probe sequences are listed next to their fluorophore and quencher.