TY - JOUR AU - Alcoser, Sergio Y. AU - Kimmel, David J. AU - Borgel, Suzanne D. AU - Carter, John P. AU - Dougherty, Kelly M. AU - Hollingshead, Melinda G. PY - 2011 DA - 2011/12/16 TI - Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts JO - BMC Biotechnology SP - 124 VL - 11 IS - 1 AB - Xenograft samples used to test anti-cancer drug efficacies and toxicities in vivo contain an unknown mix of mouse and human cells. Evaluation of drug activity can be confounded by samples containing large amounts of contaminating mouse tissue. We have developed a real-time quantitative polymerase chain reaction (qPCR) assay using TaqMan technology to quantify the amount of mouse tissue that is incorporated into human xenograft samples. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-11-124 DO - 10.1186/1472-6750-11-124 ID - Alcoser2011 ER -