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Table 1 Results from targeted sequencing libraries

From: Targeted sequencing library preparation by genomic DNA circularization

Experiment 1 2 3 4 5
Hybridization temperature (°C) 60 55 50 55 60
Number of PCR cycles 25 25 25 Direct 25
Sequencing read length (mate pair versus single) 42 42 42 42 42 by 42
Total reads 12,542,683 15,605,713 12,435,664 1,232,093 33,750,858
Mapped reads a 8,576,700 13,415,111 7,381,662 11,726 31,325,015
Captured on-target fragment-end reads c 7,560,090 11,105,527 6,330,012 8,488 30,994,237
Captured off-target reads
(as % of mapped)
1,016,610
(12%)
2,309,584
(17%)
1,051,650
(14%)
3,238
(28%)
660,937
(2%)
On-target fragment-end sequences (bases) c 4,410 4,410 4,410 4,410 8,778
Captured on-target fragment-ends with 1× (bases) c, d
(as % of on-target region-of-interest)
3,145
(71%)
3,340
(76%)
3,044
(69%)
2,809
(64%)
6,628
(75%)
Captured on-target fragment-ends with 30× (bases)b, c
(as % of on-target region-of-interest)
2,932
(66%)
3,128
(71%)
2,961
(67%)
2,160
(49%)
6,460
(73%)
Average on-target fragment-end fold-coverage 72,001 105,767 60,286 81 149,100
  1. a ELAND alignment. b Sequencing fold-coverage > 30. c Compilation of 42-base end-sequences from circularized targets. d Sequencing fold-coverage > 1.