Figure 6
From: Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions
A standard DAPI filter set can be easily modified to specifically image QDs. Since both DAPI and QDs are efficiently excited by the DAPI excitation filter and well-split by a DAPI long-pass dichroic (such as 475dcxru), the DAPI emission filter can be replaced with a specific QD 655 emission filter (such as D655/40) to isolate the emission signal from the QD alone. For greater flexibility, multiple emission filters may be mounted in an external emission filter wheel.