GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae

Table 1 f(FOA^R isolates) & recombinant fraction in SSG & gsSSG mutagenesis

PCR Fragment	Method	Frag. Size^a	Distance Ins - End^b	f(FOA^R)^c	FOA^R Tested^d	Recomb. Fract (%)^e
A	SSG	1.1	0.20	0.04	4	0
	gsSSG			1.3	5	100
B	SSG	1.0	0.12	0.0003	5	40
	gsSSG			1.9	6	100
C	SSG	1.0	0.09	0.004	4	25
	gsSSG			0.4	6	100

^a Size in kb of PCR fragment used in transformation
^b Distance in kb from marker insertion site to the fragment end nearest this site
^c Frequency of FOA^R isolates among Trp⁺ isolates
^d Number of FOA^R isolates tested by diagnostic PCR for fragment-genome recombination, each row reports on a single transformation
^e Fraction of FOA^R isolates that derived from fragment-genome recombination

ISSN: 1472-6750