Figure 2

Mutation incorporation depends on mutation-to-insertion site distance in gsSSG mutagenesis. A) Recombination between a PCR fragment and the genome incorporates the mutation when the genetic exchange (crossover) occurs at the end of the fragment (i), but does not incorporate the mutation when the genetic exchange is in the central region of the fragment (ii). B) Relationship between mutation-to-insertion distance and mutation incorporation. The mutation incorporation frequency, defined as fraction of recombinants that have incorporated the targeted mutation, is shown for transformants that contained a mutation the indicated distance from the CORE-GS insertion site. Only transformations where > 5 recombinants were analyzed are included in this graph. C) Logistic regression analysis of the same data as in B) except that all data was included regardless of the number of recombinants analyzed for a given transformation. The solid line represents the fitted logistic model, and the dashed line represents 95% confidence bands around the model.