Figure 3

The effect of PAPS formation and recycling genes on BGLS accumulation in N. benthamiana. A) Scheme of the link between formation and recycling of PAPS and the sulfotransferase reaction converting dBGLS to BGLS. Metabolites names are written in black, enzyme names are written in red, and the particular Arabidopsis isoforms used in this study are presented in parenthesis. Line arrows denote chemical conversions, while block arrows denote transport steps. B) Expression constructs coding for different PAPS formation and recycling enzymes. The term 'cTP' refers to a native CDS fragment that encodes a chloroplast targeting peptide. C) dBGLS and mdBGLS accumulation in N. benthamiana leaves expressing ORF1, ORF2, and GGP1 in combination with the indicated constructs at 6 dpi. Metabolite concentrations are expressed as peak areas from extracted ion chromatograms. D) BGLS accumulation in N. benthamiana leaves expressing ORF1, ORF2, and GGP1 in combination with the indicated constructs at 6 dpi. For both C) and D), each data point represents the mean of four biological replicates, and error bars represent standard deviations.