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Figure 1 | BMC Biotechnology

Figure 1

From: Modulation of sulfur metabolism enables efficient glucosinolate engineering

Figure 1

Metabolite analysis of BGLS-producing N. benthamiana leaves. A) Total ion chromatograms from the LC-MS analysis of N. benthamiana leaves expressing ORF1 (coding for CYP79A2 and CYP83B1) and ORF2 (coding for SUR1, UGT74B1, and AtSOT16), in the presence or absence of GGP1 (red and blue traces, respectively). A scheme of the engineered pathway is inserted, where the identities of the three main chromatogram peaks have been assigned to by dashed arrows. Metabolite abbreviations are written in black, and enzyme names are written in red. B) MS2 fragmentation patterns of the [M+Na]+ adducts of standard dBGLS (upper panel) and of the prominent compounds eluting at 6.5 min (middle panel) and 7.4 min (lower panel) in the analysis of the extracts of N. benthamiana leaves expressing ORF1, ORF2, and GGP1. The chemical structures of the proposed compounds are inserted. For mdBGLS, the location of the malonyl residue at position 6 is only tentative.

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