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Figure 4 | BMC Biotechnology

Figure 4

From: Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. colicytoplasm

Figure 4

Purification and characterization of the 4D5-8RFP recombinant protein. (A) SDS-PAGE and Coomassie stained gel of 4D5-8RFP: molecular markers (Lane 1), uninduced sample (Lane 2), IPTG induced sample (Lane 3), soluble fraction (Lane 4), flow-through Ni2+-nitrilotriacetic acid column (Lane 5), wash fraction (Lane 6), elution fractions using 500 mM imidazole (Lanes 7-10). (B) Gel filtration chromatography of standards proteins (peaks 1-4) and Ni2+-nitrilotriacetic acid enriched 4D5-8RFP protein using Sephadex G 200 gel bead column calibrated with sweet potato b-amylase 200 kDa, bovine serum albumin 66 kDa, bovine erythrocyte carbonic anhydrase 29 kDa and horse heart cytochrome c 12.4 kDa protein standards (Sigma-Aldrich).

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