Figure 3
From: Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. colicytoplasm
Plasmid maps of p4D5-8RFP used to clone and express red fluorescent antibody. The expression cassette has a T7 promoter region, a lac operator, a ribosome-binding site followed by a cloning region with NcoI and NotI restriction sites for the insertion of the DNA sequences. At the end, there is a T7 termination sequence to restrict translation to the expression of RNA for the recombinant protein. The vector also has the ampicillin resistance gene, ColE1 pBR322 origin of replication and lacI repressor gene.