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Table 6 Cytological stages of H-E stained nuclear behavior in the control and cold-shocked eggs of the loach at specific time intervals after fertilization.

From: Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

Stages

Time elapsed after fertilization (min)

  

Control

Cold-shock

  

10

20

30

40

50

60

70

80

90

100

110

120

130

Second polar body release

Female nucleus (+)

13

0

0

0

0

0

2

0

0

0

0

0

0

 

Female nucleus (-)

0

0

0

0

0

0

4

0

0

0

0

0

0

 

Irregular division

0

0

0

0

0

0

3

0

0

0

0

0

0

 

Suppression

0

0

0

0

0

0

4

0

0

0

0

0

0

2 decondensed pronuclei

 

0

10

0

0

0

0

0

0

3

0

0

0

0

1 decondensed pronucleus

 

0

6

0

0

0

0

0

3

10

0

5

0

0

First cleavage

Metaphase

0

0

14

1

0

0

0

0

0

1

0

0

0

 

Anaphase

0

0

0

16

0

0

0

0

0

4

0

0

0

 

Telophase

0

0

0

2

0

0

0

0

0

0

5

3

0

Second cleavage

Metaphase

0

0

0

0

22

0

0

0

0

0

0

0

1

 

Anaphase

0

0

0

0

0

15

0

0

0

0

0

0

1

Anuclear or Nuclear-

1-cell

0

0

0

0

0

2

3

5

8

8

4

0

11

Anuclear mosaic

2-cell

0

0

0

0

0

0

0

0

0

0

0

8

18

 

3-cell

0

0

0

0

0

0

0

0

0

0

0

0

2

Others

0

0

0

0

0

0

0

0

0

32

0

73

24

Total number of eggs observed

13

16

14

19

22

17

16

8

21

16

14

18

25

  1. 1 1-cell with anuclear nucleus with four asters.
  2. 2 1-cell embryo with a clumped nucleus.
  3. 3 Second cleavage metaphase with a clumped nucleus (2 eggs), first cleavage metaphase with a clumped nucleus and three asters (1egg), first cleavage metaphase with a clumped nucleus and four asters (3 eggs), 1-cell with two decondensed pronuclei (1 egg).
  4. 4 Second cleavage metaphase with excentric nuclear position (2 eggs).