Stages | Time elapsed after fertilization (min) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  | Control | Cold-shock | |||||||||||
 |  | 10 | 20 | 30 | 40 | 50 | 60 | 70 | 80 | 90 | 100 | 110 | 120 | 130 |
Second polar body release | Female nucleus (+) | 13 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
 | Female nucleus (-) | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
 | Irregular division | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
 | Suppression | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
2 decondensed pronuclei | Â | 0 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
1 decondensed pronucleus | Â | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 3 | 10 | 0 | 5 | 0 | 0 |
First cleavage | Metaphase | 0 | 0 | 14 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
 | Anaphase | 0 | 0 | 0 | 16 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 |
 | Telophase | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 0 |
Second cleavage | Metaphase | 0 | 0 | 0 | 0 | 22 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
 | Anaphase | 0 | 0 | 0 | 0 | 0 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Anuclear or Nuclear- | 1-cell | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 8 | 8 | 4 | 0 | 11 |
Anuclear mosaic | 2-cell | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 18 |
 | 3-cell | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Others | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 32 | 0 | 73 | 24 | |
Total number of eggs observed | 13 | 16 | 14 | 19 | 22 | 17 | 16 | 8 | 21 | 16 | 14 | 18 | 25 |