Table 5 Stages of DAPI-stained nuclear behavior in the control and cold-shocked eggs of the loach at specific time intervals after fertilization.
Stages | Time elapsed after fertilization (min) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Control | Cold-shock | ||||||||||||||
| Â | 10 | 20 | 30 | 40 | 50 | 60 | 10 | 30 | 60 | 70 | 80 | 90 | 100 | 110 | 120 | 130 |
1 condensed nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 6 | 11 | 6 | 6 | 1 | 4 | 1 | 0 |
2 condensed nuclei | 4 | 0 | 0 | 0 | 0 | 0 | 12 | 16 | 15 | 5 | 11 | 4 | 3 | 1 | 1 | 0 |
3 condensed nuclei | 4 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 8 | 3 | 0 | 1 | 0 | 0 | 0 |
2 condensed nuclei + 1 decondensed pronucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 |
1 condensed nucleus + 1 decondensed pronucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 0 | 0 | 0 |
2 decondensed pronuclei | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
1 decondensed pronucleus | 0 | 3 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 0 |
First cleavage Anaphase | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
2-cell stage | 0 | 0 | 0 | 0 | 1 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 9 |
Others 1 | 0 | 0 | 2 | 2 | 4 | 0 | 0 | 1 | 0 | 0 | 1 | 6 | 6 | 16 | 5 | 0 |
Total number of eggs observed | 8 | 9 | 7 | 7 | 7 | 11 | 20 | 20 | 21 | 26 | 32 | 25 | 14 | 21 | 12 | 9 |