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Figure 8 | BMC Biotechnology

Figure 8

From: Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

Figure 8

DAPI-stained nuclear behavior in cold-shocked eggs after fertilization. Arrows indicate condensed nuclei. Arrowheads indicate decondensed pronuclei. A (60 min after fertilization (af); just after the cold-shock treatment): two condensed nuclei, egg nucleus and sperm nucleus; B (70 min af): two condensed nuclei, sperm nucleus (sn) and egg nucleus (en) or second polar body nucleus (2 pb); C (80 min af): three condensed nuclei, sperm nucleus, egg nucleus and second polar body nucleus; D (70 min af): one decondensed male pronucleus, section from the same egg shown in E; E (70 min af): two condensed nuclei, egg nucleus and second polar body nucleus; F (90 min af): one condensed nucleus and one decondensed pronucleus; G (90 min af): two decondensed pronuclei; H (90 min af): one decondensed pronucleus. Scale bars denote 10 μm.

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