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Figure 10 | BMC Biotechnology

Figure 10

From: Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish

Figure 10

Histological sections of cold-shocked eggs after fertilization. A (70 min after fertilization: af): second polar body released and egg nucleus present just underneath the polar body; B (70 min af) second polar body present, but no egg nucleus; C (70 min af): abnormal extrusion of second polar body (note abnormal metaphase equator or nuclear substances ranging from egg surface to polar body); D (70 min af): extrusion of second polar body suppressed; E (70 min af): condensed sperm nucleus present; F (80 min af): decondensed male pronucleus appears; G (90 min af): two asters seen; H (100 min af): anaphase of first cleavage. Scale bars denote 10 μm.

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