Table 1 Sequence motifs of cis-acting elements found in uceA1
Motif sequence (letter in Figure 3) | Motif name | Motif site (from/to) | Strand | Expression |
|---|---|---|---|---|
(a) CTCC | Unnamed 4 | -293/-290 | + | Unknown |
(b) CGTGG | Unnamed1/3 | -245/-241 | - | Unknown |
(c) CGGTCA | MBS | -242/-237 | + | MYB Binding Site; Weak homology to the consensus TAACTG/CAGTTA involved in Bz2 gene activation and R and C1 factors |
(d) ATTAAT | Box 4 | -229/-224 | + | Part of the pal conserved DNA module array (pal-CMA1) involved in light responsiveness |
(e) GTCAT | Skn-1 motif | -149/-145 | + | Required for high levels of endosperm expression in cooperative interaction with others motifs (AACA, GCN4, ACGT) |
(f) ATACAAAT | ATGCAAAT-motif | -127/-120 | - | One base mismatch; Associated to the TGAGTCA motif (GCN4) separated by two bases in tandem |
(g) CAAT | CAAT-box (4) | -92/-89 | + | Core promoter; common element in promoter and enhancer regions; about 50 bp from TATA-box |
(h) CCATCTTTTT | TCA-element | -72/-63 | + | Involved in salicylic acid responsiveness; one TCA-element does not confer salicylic acid responsiveness, more than one might do it |
(i) TTATAAAA | TATA-box (19) | -44/-37 | + | Core promoter; element found from -50 to -20 of the TSS |
(j) TCTCT | Y Patch | -17/-13 | + | Core promoter; found between -100 to -1 of TSS |
(k) Pyrimidine/Purine | YR Rule | -1/+1 | + | Core promoter; Transcription Start Site (TSS) |
(l) AAAAGTTAGTTA | MBSII | -1/+11 | - | MYB binding site involved in flavonoid biosynthetic genes regulation |
(m) CGTCA | CGTCA-motif | +107/+111 | + | Involved in the MeJA-responsiveness; associated with its complementary sequence TGACG 15 bp downstream (palindrome) |
(n) TGACG | TGACG-motif | +107/+111 | - | cis-acting regulatory element involved in the MeJA-responsiveness |
(o) CTCC | Unnamed 4 | +115/+118 | - | Unknown |
(p) CACGTT | G-Box | +225/+230 | - | cis-acting regulatory element involved in light responsiveness light responsive element site associated to Box II and P-box to confer elicitor-mediated gene activation in a number of phenylpropanoid genes |
(q) CTCC | Unnamed 4 | +298/+301 | + | Unknown |
(r) AATTATTTTTTATT | AT1-motif | +316/+329 | + | Part of light responsive module |
(s) TTTCTTCTCT | 5'UTR Py-rich stretch | +328/+339 | + | Region in the 5'UTR conferring high transcription levels without the need for other upstream cis elements except for a TATA-box |
(t) CTCC | Unnamed 4 | +400/+403 | - | Unknown |
(u) ATTTTCTCCA | TC-rich repeats | +431/+439 | + | cis-acting element involved in defense and stress responsiveness |
(v) CTCC | Unnamed 4 | +435/+438 | + | Unknown |
(w) AGAAACAA | AE-box | +462/+469 | - | part of a system composed of 3 to 4 Gap-boxes and 2 AE-boxes, conferring light responsiveness |