Figure 4From: Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIIIHigh-density transfection of Hek293 cells in batch mode (without changing the medium). Cells were re-suspended in 20 mL of fresh HyQSFM4TransFx293 medium to a final density of 5 × 106 cells/mL and transfected with 2 μg DNA and 4 μg PEI in 125 mL Erlenmeyer flask at 37°C.Back to article page