Figure 1
High-density transfection of Hek293 cells. Cells were re-suspended in 20 mL of fresh HyQSFM4TransFx293 medium to a final density of 5 × 106 cells/mL and transfected with 2, 3, and 4 μg of DNA and 4, 6, and 8 μg of PEI, respectively, in 125 mL Erlenmeyer flask and 37°C. a: total cell density and viability; b: rFVIII activity measured by chromogenic assay; and c: rFVIII measured by ELISA. Values shown are the averages (mean ± SEM) of the duplicated samples from one representative experiment.