Specificity of Ab3 antibodies to 1E10-AF and 1E10-ST in sera from immunized chicken determined by ELISA. Chicken sera at different dilutions were bound to microtiter plates coated with 1E10-AF, 1E10-ST or isotype-matched control ior C5 mAb (10 μg/mL) and the reaction was developed with alkaline phosphatase-conjugated rabbit anti-chicken IgY. Sera were obtained seven days after animals received the third dose of each 1E10 mAb preparation. The data represent mean ± SD of maximal titers in chicken sera of each group.(p < 0:001, one way analysis of variance and Bonferroni multiple comparisons test). Similar letters means no difference (p > 0.05). Baseline serological antibodies were undetectable (< 100 titer).