Figure 3

The effect of the transformation with the E.coli gene gcl (lines 32, 33 and 37) or the E.coli genes gcl and hyi (lines 79, 84 and 92) on tobacco leaf phenotype. (A). The T₂ and wild type plants were grown for 35 days in CO₂-enriched cabinets (3000 μmol mol^-1) then 12 days in ambient CO₂ (350 μmol mol^-1). (B). Phenotype of line 37 transformed with E.coli gene gcl. On the left, a fully expanded leaf from an adult T₁ plant grown for 45 days in 3000 μmol.mol^-1 CO₂ and then in ambient CO₂ in a glasshouse for 10 days. On the right are three leaves from young plants grown for 12 days at 3000 μmol.mol^-1 CO2 and then 7 days in ambient CO₂.