Figure 2From: An engineered pathway for glyoxylate metabolism in tobacco plants aimed to avoid the release of ammonia in photorespirationSchematic representations of the gcl and gcl-hyi cassettes for transformation of tobacco. (A).Cassette pBIN19gcl. LB: left border; RB: right border; CaMV 35S: CaMV promoter; nos: nopaline synthase terminator; ocs octopine synthase terminator; gcl: gene encoding E. coli glyoxylate carboligase; Pnos: nopaline synthase promoter; NPTII: neophosphotransferase gene for kanamycin resistance. The arrowheads indicate the orientation of the promoter and terminator sequences. (B). Cassette pBIN19gcl-hyi. LB: left border; RB: right border; CaMV 35S: CaMV promoter; nos: nopaline synthase terminator; ocs octopine synthase terminator; gcl: gene encoding E. coli glyoxylate carboligase; hyi gene encoding E.coli hydroxypyruvate isomerase; Pnos: nopaline synthase promoter; NPTII: neophosphotransferase gene for kanamycin resistance. The arrowheads indicate the orientation of the promoter and terminator sequences.Back to article page