Figure 1

Structure of the used expression cassettes. Two expression cassettes were used in this study. The first expression cassette encodes the full-length hiAP precursor protein (aa 1- 528), including the N-terminal ER leader sequence (aa 1-19) and the C-terminal GPI anchor/cleavage site (aa 504-528). The second cassette encodes hiAP (aa 1- 503) without the GPI anchor/cleavage signal, consequently no GPI moiety can be added to the recombinant enzyme. The synthetic hiAP cDNA was flanked by a ~1 kb MTT1 promoter active sequence and the BTU2 terminator (~350 bp).