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Figure 5 | BMC Biotechnology

Figure 5

From: Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning

Figure 5

Comparison of PCR amplification products generated in typical genomic walking experiments using TAIL-PCR and FPNI-PCR methods, respectively. (A) Products generated by 3 gene-specific primers and 1-9 arbitrary degenerate primers in FT ortholog cloning of Pyracantha fortuneana using TAIL-PCR; a 510 bp fragment was obtained using the fourth AD primer, only. (B) Products generated by the same 3 gene-specific and 1-9 FP primers in FT ortholog cloning of Pyracantha fortuneana using FPNI-PCR; four specific fragments were amplified by FPNI-PCR and the longest fragment was ca. 1.7 kp. (C) Products generated by 3 gene-specific and 1-9 arbitrary degenerate (AD) primers in SOC1 ortholog cloning of Pyracantha fortuneana using TAIL-PCR. (D) Products generated by 3 gene-specific and 1-9 FP primers in SOC1 ortholog cloning of Pyracantha fortuneana using FPNI-PCR. Note: M: molecular marker; number: 1-9 arbitrary degenerate primers (FPs); -: control.

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