Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning

Figure 3

The effect of annealing temperature during the low stringency PCR cycles of the primary PCR step. Illustration of the effect of annealing temperature during the low stringency PCR cycles of the primary PCR step in FT ortholog cloning of Fragaria ananassa using FPNI-PCR (amplified products shown after the tertiary round of PCR). M: molecular marker; number in the lanes: the 1-9 FP primer; number in the bottom right corner: annealing temperature. -: control.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us