Figure 1

Schematic outline of the differences in amplification of target and non-target sequences using FPNI-PCR (a) and TAIL-PCR (b). Black and dotted lines represent the target and non-target regions, respectively. Red lines represent the region of known genomic sequence (or known sequence of T-DNA or transposon elements integrated in the genomic DNA). Each red arrow denotes a specific primer designed from the known nucleotide sequence. A blue/green chimeric arrow denotes the FP primer used in the FPNI-PCR method. Each blue arrow denotes a specific primer designed from the known adaptor sequence.