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Figure 4 | BMC Biotechnology

Figure 4

From: Rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing

Figure 4

Visual images (representative) of expression of pporRFP reporter following phytohormone or phytopathogenic bacterial treatments. After initial agroinfiltration of individual synthetic promoter construct, the tobacco leaves were re-infiltrated with (a) salicylic acid for PR1 and SARE, ethephon (an ethylene releasing chemical) for ERE, and methyl jasmonate for JAR regulatory element containing constructs or with (b) P. syringae pv. tabaci. Expression of pporRFP reporter was visualized 72 h following the treatment using epifluorescence microscope Olympus SZX12, and the images were captured using imaging software QCapture 2.56. The exposure time was 1 minute.

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