Figure 2

Fold changes of expression of pporRFP reporter following phytohormone treatments. Forty-eight hours after initial agroinfiltration of individual synthetic promoter constructs, the tobacco leaves were re-infiltrated with mock, salicylic acid (SA) for PR1 and SARE, ethephon (an ethylene releasing chemical) for ERE, and methyl jasmonate (MeJA) for JAR regulatory element containing constructs. Expression of pporRFP reporter was quantified using SPEX Fluorolog at time points 0 h (before treatment) and 72 h following treatments. Each bar represents the mean value of pporRFP expression obtained from three independent biological experiments with the standard errors of the mean noted. Significant pporRFP expression changes (indicated by asterisks) were determined statistically by use of a paired t test (p < 0.05).