Figure 1

Schematic representation of the synthetic L1ΔC22 gene and of the expressing cassettes made in the pEAQ-HT vector. (a) Synthetic L1ΔC22 gene. Unique restriction sites with nucleotide positions in brackets are shown. Structural helices and β-sheets are indicated as cylinders or white boxes, respectively. The h4 or the coil region connecting h4 and β-J where the substitutions with the M2e epitopes were made are shown in gray or black, respectively. (b) pEAQ-HT expression cassette containing the L1ΔC22 gene, under the control of the Cauliflower mosaic virus 35S promoter (CaMV-P), 5' and 3' non-translated regions (NTR) of Cowpea mosaic virus RNA-2, and nopaline synthase terminator (Nos-T). RB and LB: right and left borders of T-DNA, respectively; P19: suppressor of gene silencing; CaMV-T: CaMV terminator; NPT: neomycin phosphotransferase.