Figure 2
From: A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors
A dual expression system for induced post-translational modification of a genetically encoded biosensor. (A) Plasmid map of pUADE. P tac is an IPTG-inducible promoter and PBAD is an L-arabinose-inducible promoter. (B) Schematic representation of protein expression from pUADE in E. coli in the presence of both IPTG and L-arabinose. In the absence of L-arabinose, the PBAD promoter is inactive and no vSET is produced.