Figure 6

Total secreted cellulase in Avicel induced cultures. Cellulase secretion in T. reesei strains QM 6a and JKTR2-6 grown for 24 hours post transfer. Conidia were added to 100 ml culture medium in 250 ml Erlenmeyer flasks. Cultures were grown at 30°C, shaken at 1500 RPM. Total secreted cellulase activity was measured using the EnzChek Cellulase Substrate expressed as fluorescence/mg dry weight. Dry weights are shown in Additional File 4. The carbon sources shown are 1% glucose plus 0.5% avicel, 0.1% fructose plus 0.5% avicel, 1% glucose plus 0.5% carboxymethyl cellulose, and 0.1% fructose plus 0.5% carboxymethyl cellulose. No cellulase was detected using this detection system in either strain or condition using CMC as inducer. The data presented is based on biological triplicates that were assayed in duplicate.